To see if acidosomes contain hydrolases (digestive enzymes) we incubated cells in Gomori’s medium which in the presence of acid phosphatase and lead will form an electron-opaque reaction product. Ac...

Detail of acidosome docking sites at DV-IA planar view through the site where acidosomes are docked and/or fusing with a DV-I. These sites are labeled for the mAb specific for antigen L1. TEM taken on...

The carrier vesicles dock with the acidosomes and then fuse with these larger vesicles before or as the vesicles dock at the nascent Digestive Vacuole. Thus the contents of the carrier vesicles are fi...

The carrier vesicles dock with the acidosomes and then fuse with these larger vesicles before or as the vesicles dock at the nascent Digestive Vacuole. Thus the contents of the carrier vesicles are fi...

Quick-freeze deep-etch images show a more three dimensional view of acidosomes surrounding a phagosome. Acidosomes are unique in that they contain indentations of their membrane. The lumen has a honey...

A high magnification view of the intimate docking of acidosomes with the phagosome is particularly apparent where the P-face of the acidosome lies against the E-face of the phagosome. Numerous large I...

In more extreme cases microfilamentous material builds up into a thick layer surrounding the DV-I membrane as well as the fusion sites between the acidosomes and the DV-I. A similar thick meshwork was...

The cytopharynx/nascent digestive vacuole (phagosome) has an E-fracture face containing a high number of IMPs. Interestingly these IMPs are sometimes organized into plaques where there are several sho...

Immunogold label specific for Q2 antigen on discoidal vesicles. The same mAb used in CIL:36721 heavily labels discoidal vesicles and less heavily the cytopharyngeal membrane. A few gold particles are ...

Detail of acidosome docking sites at DV-IA planar view through the site where acidosomes are docked and/or fusing with a DV-I. These sites are labeled for the mAb specific for antigen L1. TEM taken on...